Composite

Part:BBa_K2350013:Design

Designed by: Priscilla Lee Ann Hweek   Group: iGEM17_NYMU-Taipei   (2017-10-21)


PrbcL-CrtW-CrtZ-rrnB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 420
    Illegal BglII site found at 1160
    Illegal BamHI site found at 1814
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2016


Design Notes

Use fusion pcr to combine two different important sequence (Crt-Z & Crt-W).


Source

gDNA from A.aurantiacum and operon sequence from PCC7942.

References